|
| Status |
Public on Jan 31, 2017 |
| Title |
non-segmental Vitiligo 5 |
| Sample type |
RNA |
| |
|
| Source name |
Vitiligo blood (PBMC)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Whole blood
disease state: Disease Vitiligo
|
| Treatment protocol |
RNA was extracted using Trizol reagent reagent (Invitrogen).
|
| Growth protocol |
Whole blood was drawn from vitiligo patients and healthy control subjects. Ficoll gradients (AmershamBiosciences) were used to extract peripheral blood mononuclear cells from collected blood. Specimen were immediately frozen at -80C for subsequent RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from ns vitiligo patient blood
|
| Data processing |
Background Correction: GCRMA Background Correction, Normalization: Quantile Normalization, Log Probes using Base: 2, Probeset Summarization: Median Polish, Probe filtering: skip, Probes to Import: Interrogating Probes. CEL files were imported in to Partek Genomic Suite v 6.6 (Partek, St. Louis, MO)
|
| |
|
| Submission date |
Dec 05, 2016 |
| Last update date |
Jan 31, 2017 |
| Contact name |
Rama Dey-Rao |
| E-mail(s) |
dey@buffalo.edu
|
| Phone |
716-878-3318
|
| Organization name |
University at Buffalo
|
| Department |
Dermatology
|
| Lab |
Dermatology
|
| Street address |
6086 CTRC Bldg, 875 Ellicott Street
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14203 |
| Country |
USA |
| |
|
| Platform ID |
GPL8300 |
| Series (1) |
| GSE90880 |
Expression data from blood of healthy controls and ns vitiligo patients |
|
