|
| Status |
Public on Jun 26, 2017 |
| Title |
muscle_HiraCKO_animal6475_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
tibialis anterior
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J;129S1/SvImJ
tissue: muscle, tibialis anterior
age: 6 months old
genotype: Myf6 cre/+; Hira flox/-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tibialis anterior muscles were dissected in PBS and flash frozen in liquid nitrogen. RNA was extracted using Trizol reagent.
|
| Label |
Cy3
|
| Label protocol |
5 micrograms Cy5-labelled total RNA
|
| |
|
| Hybridization protocol |
Phalanx Biotech One Array Plus protocol. Each sample was hybridized in triplicate.
|
| Scan protocol |
Agilent 0.1 XDR protocol
|
| Data processing |
Normalization was performed using median scaling normalization method
|
| |
|
| Submission date |
Dec 05, 2016 |
| Last update date |
Jun 27, 2017 |
| Contact name |
M. David Stewart |
| Organization name |
University of Houston
|
| Street address |
4800 Calhoun Rd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77204-5001 |
| Country |
USA |
| |
|
| Platform ID |
GPL13692 |
| Series (1) |
| GSE90900 |
Effect of a myofiber-specific HIRA conditional knockout on gene expression in mouse skeletal muscle at 6 months of age |
|
