|
| Status |
Public on Feb 20, 2008 |
| Title |
Mouse Liver (Random) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mouse Liver
|
| Organism |
Mus musculus |
| Characteristics |
Mouse Liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from mouse liver using Trizol reagent according to manufacturer’s instruction (Invitrogen).
|
| Label |
Cy3
|
| Label protocol |
RNA was labeled with random nanomers, CyScribe First-Strand cDNA Labeling Kit(Amersham), according to the manufacturer’s protocols.
|
| |
|
| Channel 2 |
| Source name |
Mouse Liver
|
| Organism |
Mus musculus |
| Characteristics |
Mouse Liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from mouse liver using Trizol reagent according to manufacturer’s instruction (Invitrogen).
|
| Label |
Cy5
|
| Label protocol |
RNA was labeled with random nanomers, CyScribe First-Strand cDNA Labeling Kit(Amersham), according to the manufacturer’s protocols.
|
| |
|
| |
| Hybridization protocol |
The protocols supplied by Agilent Technologies were used.
|
| Scan protocol |
The protocols supplied by Agilent Technologies were used.
|
| Description |
Custom oligo (60mer) microarray was manufactured by Agilent Technologies.
|
| Data processing |
The quantitations based on the images were performed using Feature Extraction software (Agilent Technologies). The mouse custom oligo DNA microarray chip (microarray format: 11K) contained 3896 probes that represented genes in 1948 exon-overlapping SAT (sense-antisense transcript) pairs. The same total RNA samples were reciprocally labeled with Cy3 or Cy5, hybridized to the oligo DNA on the chip, and dye-normalized. The data on all genes on the chip were used to enable the Feature Extraction software to produce the processed signals. The total mean signal on the mouse chip in each hybridization experiment was adjusted to a value of 4872.3.
|
| |
|
| Submission date |
Nov 11, 2007 |
| Last update date |
Feb 20, 2008 |
| Contact name |
Yuki Okada |
| Organization name |
Keio University
|
| Department |
Institute for Advanced Biosciences
|
| Street address |
Nipponkoku 403-1, Daihouji
|
| City |
Tsuruoka City |
| ZIP/Postal code |
997-0017 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6119 |
| Series (1) |
| GSE9581 |
Expression of Mouse and Human Sense-Antisense Transcript |
|
