|
| Status |
Public on Feb 20, 2008 |
| Title |
Human Liver (Oligo dT) |
| Sample type |
RNA |
| |
|
| Source name |
Human Liver
|
| Organism |
Homo sapiens |
| Characteristics |
Human Liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Human liver total RNA was purchased from Ambion Inc.
|
| Label |
Cy3
|
| Label protocol |
RNA was labeled using Fluorescent Direct Label Kits (oligo dT-primed labeling) (Agilent Technologies), according to the manufacturer’s protocols.
|
| |
|
| Hybridization protocol |
The protocols supplied by Agilent Technologies were used.
|
| Scan protocol |
The protocols supplied by Agilent Technologies were used.
|
| Description |
Custom oligo (60mer) microarray was manufactured by Agilent Technologies.
|
| Data processing |
The quantitations based on the images were performed using Feature Extraction software (Agilent Technologies). The same total RNA samples were labeled with single color Cy3, hybridized to the oligo DNA on the chip, and dye-normalized; the processed signals were obtained by using Feature Extraction software (Agilent Technologies). The custom oligo DNA microarray chip (microarray format: 11K) contained 2793 probes for 1486 pairs of exon-overlapping SATs. The data on all genes on the chip were used to enable the Feature Extraction software to produce the processed signals. For further analysis, the Cy3-labeled processed signal was used as the processed signal from the expression of a particular gene. The total mean signal on the human chip in each hybridization experiment was adjusted to 1491.6, so that the relative differences in gene expression could be compared among cell lines and tissues.
|
| |
|
| Submission date |
Nov 11, 2007 |
| Last update date |
Feb 20, 2008 |
| Contact name |
Yuki Okada |
| Organization name |
Keio University
|
| Department |
Institute for Advanced Biosciences
|
| Street address |
Nipponkoku 403-1, Daihouji
|
| City |
Tsuruoka City |
| ZIP/Postal code |
997-0017 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6120 |
| Series (1) |
| GSE9581 |
Expression of Mouse and Human Sense-Antisense Transcript |
|
