|
| Status |
Public on Jan 31, 2018 |
| Title |
35S::SUP-GR DEX_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
35S::SUP-GR in Ler wild-type
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: 35S::SUP-GR in Ler wild-type
treatment: DEX
phenotype: DEX treatment will lead to reduced size in organs
tissue: inflorescences with flower buds earlier than stage 10
|
| Treatment protocol |
10 µm dexamethasone diluted from a 30 mM stock in ethanol were used for the DEX treatment, while the Mock treatment was conducted with the same amount of ethanol, by inverting the plants and submerging the inflorescence for 1 min in solution containing either 10 µm DEX or ethanol, together with 0.015% Silwet L-77.
|
| Growth protocol |
The seeds of the indicated genotype were planted on soil, and grown at 22°C under continuous light conditions. The inflorescence with flower buds earlier than stage 10 was sampled from Mock and DEX-treated plants.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and the RNAprotect reagent (Qiagen) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed following NimbleGen standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed following NimbleGen standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed following NimbleGen standard operating protocol. See www.nimblegen.com.
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Dec 22, 2016 |
| Last update date |
Jan 31, 2018 |
| Contact name |
Yifeng XU |
| E-mail(s) |
yifeng@tll.org.sg
|
| Phone |
68727742
|
| Organization name |
Temasek Life Sciences Laboratory
|
| Street address |
1 Research Link
|
| City |
Singapore |
| State/province |
- |
| ZIP/Postal code |
117604 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL13970 |
| Series (1) |
| GSE92729 |
Gene expression analysis of the inflorescence of 35S::SUP-GR after Mock and DEX treatment |
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