|
| Status |
Public on Feb 26, 2010 |
| Title |
CL-AG13153-II-30yrs |
| Sample type |
RNA |
| |
|
| Source name |
Fibroblast, NIA Repository, Coriell
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male, Karyotype: normal
|
| Biomaterial provider |
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=AG13153
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from the cell lysate using Qiagen RNeasy mini kit according to the manufacturer’s description.
|
| Label |
Biotin
|
| Label protocol |
http://www.appliedmicroarrays.com/pdf/iexpress2006_web.pdf
|
| |
|
| Hybridization protocol |
http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
|
| Scan protocol |
Slides were scanned at 5 µm resolution with a ScanArray 4000xl (Perkin Elmer)
|
| Description |
Culture Preparation Day 1: Cells were seeded at 9000-12000 cells per cm2. Day 2: Change to starvation medium (EMEM with 0.2% BSA and 1% L-glutamine) and incubation for 24 hours; Day 3: Aspirate the starvation medium and wash the cells with ice cold 1 x PBS. Use Qiagen lysis buffer (RLT) to collect the cell lysates and store at -80 °C.
|
| Data processing |
global median normalization, CodeLink Expression Analysis 4.1.0.4163
|
| |
|
| Submission date |
Nov 21, 2007 |
| Last update date |
Mar 05, 2010 |
| Contact name |
Andres Kriete |
| Organization name |
Drexel University
|
| Department |
School of Biomedical Engineering
|
| Street address |
3141 Chestnut St
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
|
