|
| Status |
Public on Apr 21, 2017 |
| Title |
Mock, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HuH7 cells transfected with mock and measured after 24h
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: mock
|
| Treatment protocol |
24h after seeding (50%-70% confluence) HuH7 cells were transfected with 10nM antisense oligonucleotide or mock in 6 well plates using lipofectamine (5 ug/ml). After 4 hours media was changed and cells incubated for 20h without antisense oligonucleotide or mock.
|
| Growth protocol |
HuH7 cells were seeded in 6 well plates with 2,5x10^5 cells/ml in 2ml
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were homogenized in Trizol and RNA was extracted according to standard protocols.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cDNA were prepared and hybridized using the GeneChip Hybridization, Wash, and Stain Kit (Affymetrix)
|
| Scan protocol |
GCS3000 (Affymetrix)
|
| Data processing |
The raw cel files were converted into probeset-specific expression values using Robust Multiarray Averaging (Bioconductor package Affy) with summarization method using Brainarray cdf annotations.
|
| |
|
| Submission date |
Jan 06, 2017 |
| Last update date |
Apr 21, 2017 |
| Contact name |
Peter H Hagedorn |
| E-mail(s) |
peter.hagedorn@roche.com
|
| Organization name |
Roche Innovation Center Copenhagen A/S
|
| Department |
Research
|
| Lab |
Bioinformatics
|
| Street address |
Fremtidsvej 3
|
| City |
Horsholm |
| State/province |
Danmark |
| ZIP/Postal code |
DK2970 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL19145 |
| Series (1) |
| GSE93245 |
Expression data from transfection of two different antisense oligonucleotides in HuH7 cells |
|
