|
| Status |
Public on Nov 30, 2007 |
| Title |
Heart_iron_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
cardiac muscle, iron diet
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6.
Gender: male.
Age: 18 weeks.
Tissue: cardiac muscle.
|
| Biomaterial provider |
Laboratory animal centre of the University of Oulu
|
| Growth protocol |
iron diet:control diet supplemented with 2% carbonyl iron
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy RNA isolation kit including RNase-free DNase treatment (Qiagen), according to the manufacturer's protocol (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Using Illumina TotalPrep RNA Amplification kit (#IL1791, Ambion), following the menufacturer's protocol. Input amount: 200ng of total RNA. Incubation of labeling reaction: 14h.
|
| |
|
| Hybridization protocol |
Using Illumina reagents and protocols. Hybridization: 18h at 55 degrees C.
|
| Scan protocol |
Standard scan factor in Illumina BeadArray reader.
|
| Description |
Strain: C57BL/6.
Gender: male.
Age: 18 weeks.
Tissue: cardiac muscle.
Third biological replicate of iron group
|
| Data processing |
Array data were normalized with Inforsense KDE version 2.0.4 using quantile normalization method.
|
| |
|
| Submission date |
Nov 28, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Alejandra Rodriguez-Martinez |
| E-mail(s) |
alejandra.rodriguez.martinez@uta.fi
|
| Organization name |
University of Tampere
|
| Department |
Institute of Biomedical Technology
|
| Street address |
Biokatu 6
|
| City |
Tampere |
| ZIP/Postal code |
33520 |
| Country |
Finland |
| |
|
| Platform ID |
GPL4234 |
| Series (1) |
| GSE9726 |
Effects of iron overload on gene expression in the cardiac and skeletal muscle of mice |
|
