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Sample GSM245434 Query DataSets for GSM245434
Status Public on Jun 03, 2008
Title human cellular RNA_primary airway epithelial cells_tracheobronchial _H510_2h_1mM_HG-U133A_2_YCHSUP501
Sample type RNA
 
Source name Sample Type: human cellular RNA, Cell Line: primary airway epithelial cells, Cell Type: tracheobronchial
Organism Homo sapiens
Characteristics Additional Information: please return the leftover samples to PI.
Array Type: HG-U133A_2
Amount to Core: 12
Units of Amount: ug
OD260: 19.666
Ratio: 2.14
Biomaterial provider Yin Chen, CIIT Centers for Health Research, 6 Davis Drive, Research Triangle Park, NC, 27709, yin chen, yinchen@ciit.org, 558-1320
Growth protocol Growth Media: C media, Growth Conditions: 37C, 5%CO2
Extracted molecule total RNA
Extraction protocol Extraction Method: Rneasy mini-kit
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description H510_2h_1mM_HG-U133A_2_YCHSUP501: Primary epithelial cells from airways of four different healthy individuals were cultivated and grown to full differentiation. Apical sides of the cells were treated with HCLO at different doses (0, 0.4,1, 4mM) for 2 and 6hrs respectively. Total RNA was subjuect to transcriptional analysis.
Data processing Affymetrix GCOS 1.0
 
Submission date Nov 28, 2007
Last update date Aug 14, 2011
Contact name Yin Chen
E-mail(s) yinchen@thehamner.org
Phone 919-558-1320
Organization name Hamner Institutes for Health Sciences
Department Division of Translational Biology
Street address 6 Davis Dr.
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL571
Series (1)
GSE11630 HOCL induced airway epithelial gene expression

Data table header descriptions
ID_REF Affymetrix probe name
VALUE GCOS-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 192.093 P 0.000662
AFFX-BioB-M_at 259.946 P 4.4e-05
AFFX-BioB-3_at 185.87 P 0.000225
AFFX-BioC-5_at 219.767 P 0.00039
AFFX-BioC-3_at 228.121 P 5.2e-05
AFFX-BioDn-5_at 1560.9 P 4.4e-05
AFFX-BioDn-3_at 2991.07 P 9.5e-05
AFFX-CreX-5_at 6958.38 P 5.2e-05
AFFX-CreX-3_at 9258.14 P 4.4e-05
AFFX-DapX-5_at 994.343 P 4.4e-05
AFFX-DapX-M_at 1638.21 P 0.000127
AFFX-DapX-3_at 2028.13 P 4.4e-05
AFFX-LysX-5_at 141.383 P 5.2e-05
AFFX-LysX-M_at 129 P 0.000509
AFFX-LysX-3_at 284.891 P 7e-05
AFFX-PheX-5_at 214.735 P 4.4e-05
AFFX-PheX-M_at 316.541 P 4.4e-05
AFFX-PheX-3_at 213.043 P 5.2e-05
AFFX-ThrX-5_at 205.011 P 4.4e-05
AFFX-ThrX-M_at 389.476 P 4.4e-05

Total number of rows: 22277

Table truncated, full table size 646 Kbytes.




Supplementary file Size Download File type/resource
GSM245434.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table
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