Additional Information: please return the leftover samples to PI. Array Type: HG-U133A_2 Amount to Core: 12 Units of Amount: ug OD260: 11.442 Ratio: 2.08
Biomaterial provider
Yin Chen, CIIT Centers for Health Research, 6 Davis Drive, Research Triangle Park, NC, 27709, yin chen, yinchen@ciit.org, 558-1320
Growth protocol
Growth Media: C media, Growth Conditions: 37C, 5%CO2
Extracted molecule
total RNA
Extraction protocol
Extraction Method: Rneasy mini-kit
Label
biotin
Label protocol
standard Affymetrix protocol
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
H421_2h_4mM_HG-U133A_2_YCHSUP501: Primary epithelial cells from airways of four different healthy individuals were cultivated and grown to full differentiation. Apical sides of the cells were treated with HCLO at different doses (0, 0.4,1, 4mM) for 2 and 6hrs respectively. Total RNA was subjuect to transcriptional analysis.