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Status |
Public on Apr 10, 2017 |
Title |
TPK423 |
Sample type |
RNA |
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Source name |
T47D-Y cells stably expressing empty pIRESneo3 plasmid, co-treated with progesterone and aglepristone for 6 hours
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Organism |
Homo sapiens |
Characteristics |
parental cell line: T47D-Y tissue: Breast stable vector integration: pIRESneo3-empty treatment: progesterone and aglepristone time point: 6 hr
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Treatment protocol |
Samples treated in IMEM medium, with or without progestin (10e-8 M) and/or antiprogestin (10e-7 M) for 6 hr.
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Growth protocol |
Samples grown in complete medium (MEM, 5% FBS, 1% NEAA, 1% pen/strep, 6ng/ml insulin, 200 ug/ml G418) before 1 day starvation in IMEM medium, then treated in IMEM medium for 6 hr.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
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|
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Hybridization protocol |
Standard Illumina hybridization protocol
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Scan protocol |
Standard Illumina scanning protocol
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Description |
T47D_PR_null_progesterone_aglepristone_6h_expt_2_rep_2
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Data processing |
The data were filtered and any probes with a detection P value less than 0.05 were removed. The data was then normalized using log2 transformation and quantile normalization with R using the lumi bioconductor package
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Submission date |
Feb 01, 2017 |
Last update date |
Apr 10, 2017 |
Contact name |
Todd P Knutson |
E-mail(s) |
knut0297@umn.edu
|
Phone |
612-626-8911
|
Organization name |
University of Minnesota
|
Department |
Minnesota Supercomputing Institute
|
Street address |
117 Pleasant St SE
|
City |
Minneapolis |
State/province |
MN |
ZIP/Postal code |
55455 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE94363 |
Posttranslationally modified progesterone receptors direct ligand-specific expression of breast cancer stem cell-associated gene programs |
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