|
| Status |
Public on May 02, 2018 |
| Title |
Treated-HSF16,Sensitive 1431005386_83 |
| Sample type |
RNA |
| |
|
| Source name |
Patient-derived Fibroblast cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Fibroblast cell line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified with the RNeasy Mini-kit (Qiagen, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 400ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 and performed by the Mayo Clinic Genomic Core
|
| Description |
Gene expression data from patient derived fibroblast cell lines
Technical replicates 1
|
| Data processing |
The data were analyzed with using Partek Genomic Suite Ver. 6.6 (Partek Inc, St. Louis, MO) Partek Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 23, 2017 |
| Last update date |
May 03, 2018 |
| Contact name |
Michael K Asiedu |
| E-mail(s) |
makasiedu@yahoo.com
|
| Phone |
5072843482
|
| Organization name |
Mayo Foundation for Medical Education and Research
|
| Department |
Surgery
|
| Street address |
200 Fisrt ST SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE95298 |
Patient- and Cell Type-Specific Heterogeneity of Metformin Response. |
|
