|
| Status |
Public on May 02, 2018 |
| Title |
BT20 Treated |
| Sample type |
RNA |
| |
|
| Source name |
commercial cell lines
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Cancer cell lines
cell line: BT20
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified with the RNeasy Mini-kit (Qiagen, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 400ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 and performed by the Mayo Clinic Genomic Core
|
| Description |
Gene expression data from patient derived fibroblast cell lines
BT20 BT20 Treated
|
| Data processing |
The data were analyzed with using Partek Genomic Suite Ver. 6.6 (Partek Inc, St. Louis, MO) Partek Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 23, 2017 |
| Last update date |
Dec 17, 2018 |
| Contact name |
Michael K Asiedu |
| E-mail(s) |
makasiedu@yahoo.com
|
| Phone |
5072843482
|
| Organization name |
Mayo Foundation for Medical Education and Research
|
| Department |
Surgery
|
| Street address |
200 Fisrt ST SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE95298 |
Patient- and Cell Type-Specific Heterogeneity of Metformin Response. |
|
| Relations |
| Reanalyzed by |
GSE123917 |
