|
| Status |
Public on Feb 01, 2008 |
| Title |
RS4;11_untreated_3h_technical replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
RS4;11_untreated_3h
|
| Organism |
Homo sapiens |
| Characteristics |
Ph- cell line
|
| Biomaterial provider |
German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany)
|
| Treatment protocol |
none
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction followed by purification using RNeasy
|
| Label |
Cy3
|
| Label protocol |
Samples were labeled using the CyScribe Post-labeling Kit (Amersham Biosciences)
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Human Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
RNA pooled from ten human cell lines
|
| Biomaterial provider |
Stratagene
|
| Treatment protocol |
none
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
None
|
| Label |
Cy5
|
| Label protocol |
Samples were labeled using the CyScribe Post-labeling Kit (Amersham Biosciences)
|
| |
|
| |
| Hybridization protocol |
Arrays were hybridized and washed as described in Håkansson et al. (Genes Chromosomes Cancer 2008)
|
| Scan protocol |
Arrays were scanned in an Agilent Microarray scanner
|
| Description |
cDNA microarray data
|
| Data processing |
Image analysis was performed using Gene Pix v4.0. Result-files were uploaded into BASE where they were normalized using pin-based Lowess and filtered to keep spots with a signal to noise ratio >1.5
|
| |
|
| Submission date |
Jan 25, 2008 |
| Last update date |
Jan 31, 2008 |
| Contact name |
Petra Johnels |
| E-mail(s) |
petra.johnels@med.lu.se, thoas.fioretos@med.lu.se
|
| Phone |
+46 46 173398
|
| Fax |
+46 46 131061
|
| Organization name |
Lund University
|
| Department |
Clinical Genetics
|
| Street address |
Lund University Hospital
|
| City |
Lund |
| ZIP/Postal code |
221 85 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL4861 |
| Series (1) |
| GSE10283 |
BCR/ABL1-Dependent Transcriptional Response Reveals Enrichment for Genes Involved in Negative Feedback Regulation |
|
