|
Status |
Public on Feb 01, 2008 |
Title |
RS4;11_untreated_3h_technical replicate 2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Stratagene Universal Human Reference RNA
|
Organism |
Homo sapiens |
Characteristics |
RNA pooled from ten human cell lines
|
Biomaterial provider |
Stratagene
|
Treatment protocol |
none
|
Growth protocol |
none
|
Extracted molecule |
total RNA |
Extraction protocol |
None
|
Label |
Cy3
|
Label protocol |
Samples were labeled using the CyScribe Post-labeling Kit (Amersham Biosciences)
|
|
|
Channel 2 |
Source name |
RS4;11_-_3h_DS
|
Organism |
Homo sapiens |
Characteristics |
Ph- cell line
|
Biomaterial provider |
German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany)
|
Treatment protocol |
none
|
Growth protocol |
none
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction followed by purification using RNeasy
|
Label |
Cy5
|
Label protocol |
Samples were labeled using the CyScribe Post-labeling Kit (Amersham Biosciences)
|
|
|
|
Hybridization protocol |
Arrays were hybridized and washed as described in HÃ¥kansson et al. (Genes Chromosomes Cancer 2008)
|
Scan protocol |
Arrays were scanned in an Agilent Microarray scanner
|
Description |
cDNA microarray data
|
Data processing |
Image analysis was performed using Gene Pix v4.0. Result-files were uploaded into BASE where they were normalized using pin-based Lowess and filtered to keep spots with a signal to noise ratio >1.5
|
|
|
Submission date |
Jan 25, 2008 |
Last update date |
Jan 31, 2008 |
Contact name |
Petra Johnels |
E-mail(s) |
petra.johnels@med.lu.se, thoas.fioretos@med.lu.se
|
Phone |
+46 46 173398
|
Fax |
+46 46 131061
|
Organization name |
Lund University
|
Department |
Clinical Genetics
|
Street address |
Lund University Hospital
|
City |
Lund |
ZIP/Postal code |
221 85 |
Country |
Sweden |
|
|
Platform ID |
GPL4861 |
Series (1) |
GSE10283 |
BCR/ABL1-Dependent Transcriptional Response Reveals Enrichment for Genes Involved in Negative Feedback Regulation |
|