|
| Status |
Public on Jul 05, 2019 |
| Title |
Cirrhotic_Pt-181_gene_expression |
| Sample type |
RNA |
| |
|
| Source name |
Non-tumor cirrhotic liver
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Non-tumor cirrhotic liver
pathologic classification: Cirrhosis
tissue preparation: FFPE
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed on four to six macrodissected sections (5 μm) from each FFPE block using the automated procedure QIAcube and QIAGEN spin columns (Qiagen, Hilden, Germany). Total RNA (500ng) was used for whole-genome gene-expression profiling.
|
| Label |
biotin
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Description |
Sample name: NY2177
RNA extracted from non-tumoral cirrhotic tissue was genotyped with HumanHT-12 WG-DASL V4.0 R2 expression beadchip (Illumina)
|
| Data processing |
Raw data were normalized using cubic spline algorithm implemented in the Illumina Normalizer module of the GenePattern analysis toolkit (www.broadinstitute.org/genepattern) as previously described (Sia D et al, Gastroenterology, 2013).
|
| |
|
| Submission date |
May 07, 2017 |
| Last update date |
Jul 05, 2019 |
| Contact name |
Sara Torrecilla |
| Organization name |
IDIBAPS
|
| Lab |
Liver Cancer Translational Research Laboratory
|
| Street address |
Rossello Street, 149
|
| City |
Barcelona |
| State/province |
Catalonia |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL14951 |
| Series (2) |
| GSE98617 |
Trunk events present minimal intra- and inter-tumoral heterogeneity in hepatocellular carcinoma [gene expression] |
| GSE98620 |
Trunk events present minimal intra- and inter-tumoral heterogeneity in hepatocellular carcinoma |
|
