|
| Status |
Public on Oct 06, 2020 |
| Title |
Tax1BP1 knock-out 2 |
| Sample type |
RNA |
| |
|
| Source name |
Tax1BP1 knock-out
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: Tax1BP1 knock-out
age: 12 weeks
Sex: male
tissue: liver
|
| Treatment protocol |
no treatment
|
| Growth protocol |
Mice were housed under 12 h day/night cycles, were fed standard rodent chow and had access to drinking water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the RNeasy Mini Kit from Qiagen.RNA was amplified using the Ovation Pico WTA System V2 from NUGEN applied for 50ng total RNA
|
| Label |
biotin
|
| Label protocol |
Samples were labelled with Encore biotin module (NUGEN) washed and stained using the HWS Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Mouse Gene 1.0 ST arrays (Affymetrix) were hybridized with 2.5 mg labelled cDNA.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
75_(MoGene-1_0-st-v1).CEL
|
| Data processing |
Statistical analysis was done with the statistical computing environment R. Additional software packages (affy, geneplotter, multtest, and vsn) were taken from the Bioconductor project. Microarray preprocessing: Probe level normalization was conducted using the variance stabilization method (vsn) from Huber et al..
probe group file: Unsupported Mouse Gene 1.0 ST Array CDF Files
meta-probeset file: MoGene-1_0-st-v1 Probeset Annotations, CSV Format, Release 32 (7.8 MB, 10/7/11)
|
| |
|
| Submission date |
May 15, 2017 |
| Last update date |
Oct 06, 2020 |
| Contact name |
Claudia Döring |
| E-mail(s) |
C.Doering@em.uni-frankfurt.de
|
| Organization name |
Johann Wolfgang-Goethe University Frankfurt a.M.
|
| Department |
Medical School, Institite of Pathology
|
| Street address |
Theodor Stern Kai 7
|
| City |
Frankfurt |
| ZIP/Postal code |
60590 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE98908 |
Differential expression of mRNA in Tax1BP1 knock-out mice [mRNA] |
| GSE98909 |
Differential expression of miRNA and mRNA in Tax1BP1 knock-out mice |
|
