|
Status |
Public on Aug 12, 2017 |
Title |
AB81, hypoxia, HIF12kd, b |
Sample type |
RNA |
|
|
Source name |
conditionally immortalized human podocytes (AB81)
|
Organism |
Homo sapiens |
Characteristics |
condition: hypoxia genotype: HIF12kd
|
Treatment protocol |
Differentiated human podocytes were treated with hypoxia (1% O2) for 24h.
|
Growth protocol |
Conditionally immortalized human podocytes were cultured under permissive conditions (33°C) in RPMI-1640 supplemented with 10% FBS, penicillin (100 U/ml)/streptomycin (100 µg/ml), and 1% ITS. Differentiation was induced by thermoshift to 37°C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using Rneasy kit (Qiagen) following manufacturer's protocol
|
Label |
biotin
|
Label protocol |
Fragmentation and biotin labelling of each amplified cDNA was processed with the NuGen Encore Biotin Module (NuGen Inc.) and according to manufacturer's protocol.
|
|
|
Hybridization protocol |
standard Affymetrix protocol
|
Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
Description |
Gene expression data from conditionally immortalized podocytes (AB81)
|
Data processing |
The raw data was normalized using Robust Multichip Algorithm (RMA) using CustomCDF v 18 ENTREZG
|
|
|
Submission date |
May 25, 2017 |
Last update date |
Jan 23, 2018 |
Contact name |
Felix Eichinger |
E-mail(s) |
eichinge@umich.edu
|
Organization name |
The University of Michigan
|
Department |
Division of Nephrology
|
Street address |
1150 W. Medical Center Drive
|
City |
Ann Arbor |
State/province |
Michigan |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL19109 |
Series (2) |
GSE99323 |
Transcriptome-based network analysis reveals renal cell type-specific dysregulation of hypoxia-associated transcripts [AB81] |
GSE99340 |
Transcriptome-based network analysis reveals renal cell type-specific dysregulation of hypoxia-associated transcripts |
|