|
| Status |
Public on Aug 18, 2017 |
| Title |
Wap-Cre:PTEN tumor PW83 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Mouse Mammary Tumor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mammary tumor
histology: Poorly differentiated
sample dimension: Mammary Tumor less than 1.0 cm diameter
|
| Treatment protocol |
Total DNA obtained from primary tumors were used for arrayCGH analysis to determine changes in copy number
|
| Growth protocol |
Samples from independent primary mammary tumors were frozen and kept in -80 degree.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA was extracted using QIAGEN DNeasy Blood & Tissue Kits
|
| Label |
Cy5
|
| Label protocol |
Mouse 1X1M CGH experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| |
|
| Channel 2 |
| Source name |
pooled mouse DNA for reference
|
| Organism |
Mus musculus |
| Characteristics |
sample type: pooled mouse DNA
|
| Treatment protocol |
Total DNA obtained from primary tumors were used for arrayCGH analysis to determine changes in copy number
|
| Growth protocol |
Samples from independent primary mammary tumors were frozen and kept in -80 degree.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA was extracted using QIAGEN DNeasy Blood & Tissue Kits
|
| Label |
Cy3
|
| Label protocol |
Mouse 1X1M CGH experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| |
|
| |
| Hybridization protocol |
Mouse 1X1M CGH experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| Scan protocol |
Mouse 1X1M CGH experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| Description |
PW83
|
| Data processing |
Mouse 1X1M CGH experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
normalized values are represented by normalized log10 ratio (Cy5/Cy3) representing test/reference
|
| |
|
| Submission date |
May 31, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Sharon Wang |
| Organization name |
University of Toronto
|
| Street address |
67 College Street Room 456
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 2M1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL10448 |
| Series (2) |
| GSE99495 |
microRNA-143/145 loss induces Ras-signaling and cooperates with Pten-deficiency to promote basal-like breast cancer [CGH] |
| GSE99496 |
microRNA-143/145 loss induces Ras-signaling and cooperates with Pten-deficiency to promote basal-like breast cancer |
|
