|
| Status |
Public on Oct 03, 2004 |
| Title |
A549 TNF+Stretch Oct 20 2002 |
| Sample type |
RNA |
| |
|
| Source name |
alveolar epithelial cell line exposed to TNF (20ng/ml) and cyclic stretch (20% elongation) for 1hr
|
| Organism |
Homo sapiens |
| Extracted molecule |
total RNA |
| |
|
| Description |
A549 a pulmonary alveolar epithelial cell line was used in mechanical stretch and parenchymal cell activation experiments. Cells were grown in flexercell plates coated with collagen I, and allowed to grow O/N. Cells were then exposed to TNF alpha 20ng/ml stimulation + cyclic stretch (20% elongation) for 1hr or 4hrs. Two replicates for each time point were performed. This is the first replicate for the 1hr time point. Experiemnt was carried out Oct 20 2002
Keywords = A549 cells RNA
Keywords = mechanical stretch
|
| |
|
| Submission date |
Jul 04, 2004 |
| Last update date |
May 27, 2005 |
| Contact name |
Claudia Chimisso dos Santos |
| E-mail(s) |
dossantosc@smh.toronto.on.ca
|
| Phone |
(416)340-4800 ext 6596
|
| Fax |
(416) 340-4638
|
| Organization name |
Toronto General Research Institute
|
| Department |
Critical Care
|
| Lab |
Thoracic Surgery Research
|
| Street address |
200, Elizabeth Street
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1C4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL201 |
| Series (1) |
| GSE1541 |
A549 parenchymal cell activation |
|
