|
| Status |
Public on May 23, 2018 |
| Title |
BL2-2 [Affymetrix] |
| Sample type |
RNA |
| |
|
| Source name |
BL2 cells
|
| Organism |
Homo sapiens |
| Characteristics |
agent: unstimulated
cell line: BL2
|
| Treatment protocol |
For stimulation studies, cells were cultured in cell culture medium supplemented with 10 mM HEPES at 1 x 10^6 cells/ml and incubated with B-cell activating factor (BAFF) for up to 24 hrs.
|
| Growth protocol |
Cells were maintained in RPMI 160 supplemented with 10% fetal calf serum (FCS) and glutamine; peniciline; streptomycine at cell densities between 2x10^5 and 1x10^6 cells per ml.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with RNAeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
RNA was labelled using Affymetrix GeneChip IVT Labelling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Fragmentation and hybridization on Human ST1.0 Arrays were processed according to manufacturer's recommendations by the TAL (UMG, Germany)
|
| Scan protocol |
Affymetrix protocol
|
| Data processing |
R/Bioconductor
|
| |
|
| Submission date |
Jun 16, 2017 |
| Last update date |
May 23, 2018 |
| Contact name |
Alexander Wolff |
| E-mail(s) |
alexander.wolff@med.uni-goettingen.de
|
| Organization name |
UMG
|
| Department |
Medical Statistics
|
| Lab |
Beissbarth Group
|
| Street address |
Humboldtallee 32
|
| City |
Goettingen |
| State/province |
Lower Saxony |
| ZIP/Postal code |
37073 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17556 |
| Series (2) |
| GSE100111 |
B-cell activating factor (BAFF) stimulation of Burkitt Lymphoma cell line [Affymetrix] |
| GSE100112 |
B-cell activating factor (BAFF) stimulation of Burkitt Lymphoma cell line |
|
