|
| Status |
Public on Apr 18, 2018 |
| Title |
Sample2468_BCellData |
| Sample type |
RNA |
| |
|
| Source name |
purified peripheral blood CD19+ B cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
cell type: CD19+ B cells
first_diagnosis: Rheumatoid Arthritis
working_diagnosis: Rheumatoid Arthritis
Sex: M
|
| Treatment protocol |
No ex vivo treatment
|
| Growth protocol |
Peripheral blood mononuclear cells first isolated by density centrifugation on Lymphoprep (Axis-Shield Diagnostics, Dundee, UK) underwent positive selection using anti-CD19 magnetic microbeads (Miltenyi Biotec, Bergisch, Gladback, Germany); median cell purity was 96.4% by flow cytometry. Total B lymphocyte RNA was immediately extracted using the AllPrep DNA/RNA Mini kit (Qiagen GmbH, Germany), then quality controlled using an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, California, USA). The median RNA integrity number of samples used was 9.4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
AllPrep RNA extraction kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
cRNA generated from 250ng total RNA using Illumina TotalPrep RNA Amplification kit
|
| |
|
| Hybridization protocol |
Illumina HT12 BeadChip
|
| Scan protocol |
Illumina iScan
|
| Description |
3999319005_L_Grn.idat
Freshly isolated and purified peripheral blood CD19+ B cells - Rheumatoid Arthritis
|
| Data processing |
Normalisation via VST (Variance Stabilising Transformation and RSN (Robust Spline Normalisation)
|
| |
|
| Submission date |
Jun 29, 2017 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Arthur Pratt |
| E-mail(s) |
arthur.pratt@newcastle.ac.uk
|
| Organization name |
Newcastle University
|
| Department |
Faculty of Medical Sciences
|
| Lab |
Musculoskeletal Research Group
|
| Street address |
Framlington Place
|
| City |
Newcastle upon Tyne |
| ZIP/Postal code |
NE2 4HH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE100648 |
Early arthritis B cell gene expression profiling |
|
