|
| Status |
Public on Jun 30, 2020 |
| Title |
glioblastoma multiforme tissue 3 |
| Sample type |
RNA |
| |
|
| Source name |
glioblastoma multiforme tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: brain
|
| Treatment protocol |
All tissues were collected right after resection in the operating room and quickly frozen in liquid nitrogen for 2-3 hours and stored at -80 °C.
|
| Growth protocol |
-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA by using Ambion® WT Expression Kit.
|
| |
|
| Hybridization protocol |
Following labeling, 5.5 ug of cDNA were hybridized for 16 hr at 45°C on GeneChip Human Transcriptome Array 2.0 in Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned by using Affymetrix® GeneChip Command Console (AGCC) which installed in GeneChip® Scanner 3000 7G.
|
| Description |
LncRNA and mRNA expression profiles from glioblastoma multiforme tissue
|
| Data processing |
The data were analyzed with Robust Multichip Analysis (RMA) algorithm using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 30, 2017 |
| Last update date |
Jun 30, 2020 |
| Contact name |
Yali Zhou |
| Organization name |
Nanjing University
|
| Street address |
22 Hankou Road
|
| City |
Nanjing |
| ZIP/Postal code |
210093 |
| Country |
China |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE100675 |
LncRNA and mRNA expression data from human glioblastoma multiforme |
|
