|
| Status |
Public on Jul 07, 2018 |
| Title |
mammary gland tissue C13 |
| Sample type |
RNA |
| |
|
| Source name |
mammary gland tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: mammary gland
subtype: non-tumor breast tissues
gender: female
|
| Treatment protocol |
Tissues from surgury and promptly frozen in liquid nitrogen.
|
| Growth protocol |
breast cancer and non-tumor breast tissues
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Total RNA from each sample was amplified and transcribed into fluorescent cRNA utilizing ramdom primer according to Arraystar Super RNA Labeling protocol (Arraystar, Inc.)
|
| |
|
| Hybridization protocol |
The labeled cRNAs were hybridized onto the Arraystar Human circRNA Arrays (8x15K, Arraystar), and incubated for 17 hours at 65℃ in an Agilent Hybridization Oven.
|
| Scan protocol |
After washing, slides were scanned with the Agilent Scanner G2505C.
|
| Data processing |
A series of data processing including quantile normalization were performed using the R software package. The circRNAs that at least 4 out of 11 tissue samples have flags in P or M (defined by GeneSpring software) were retained for further differential analyses.Differentially expressed circRNAs with statistical significance between two samples or two groups were identified using fold change cutoff or through Volcano Plot filtering respectively.
|
| |
|
| Submission date |
Jul 11, 2017 |
| Last update date |
Jul 07, 2018 |
| Contact name |
Jianzhen Xu |
| E-mail(s) |
jzxu01@stu.edu.cn
|
| Phone |
+86-0754-88900491
|
| Organization name |
Shantou University Medical College
|
| Street address |
No.22 Rd Xinling
|
| City |
Shantou |
| ZIP/Postal code |
515041 |
| Country |
China |
| |
|
| Platform ID |
GPL19978 |
| Series (2) |
| GSE101123 |
circRNA expression in breast cancer and non-tumor breast tissues |
| GSE101124 |
circRNA expression in breast cancer |
|
