|
| Status |
Public on Mar 01, 2018 |
| Title |
Mim6_24h_L_Midbrain |
| Sample type |
RNA |
| |
|
| Source name |
Left midbrain from brain Microcebus murinus 24h after CAV-2 infection, contralateral side
|
| Organism |
Microcebus murinus |
| Characteristics |
gender: female
age: 2 year old
cav-2 infection helper dependent 109 physical particles: Contralateral to CAV-2 infection
brain tissue: Midbrain
|
| Growth protocol |
Animals were all born in captivity in our breeding colony (CECEMA, University of Montpellier, France). 12 animals were used in this study. All animal experiments were conducted in strict accordance with the European Union Directive (2010/63/EU) for the care and the use of laboratory animals (Animal authorization CE-LR-0502, ethical committee of the Languedoc-Roussillon, France).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using Rneasy mini Kit (QIAGEN, Santa Clarina, CA) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 1 à 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Agilent Scanner 3000.
|
| Description |
Gene expression changes induced by CAV-2 in primate midbrain, 24h after infection
|
| Data processing |
he data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Aug 16, 2017 |
| Last update date |
Mar 01, 2018 |
| Contact name |
Gina DEVAU |
| E-mail(s) |
gina.devau@umontpellier.fr
|
| Phone |
33 467144830
|
| Organization name |
Inserm U1198-UM-EPHE
|
| Department |
Universite de Montpellier CC105
|
| Lab |
Mecanismes Moleculaires dans les Demences Neurologiques (MMDN)
|
| Street address |
Place Eugene Bataillon
|
| City |
Montpellier |
| ZIP/Postal code |
34095 cedex5 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE102709 |
Gene expression changes in the midbrain of Microcebus murinus induced by Canine adenovirus type 2 (CAV-2) |
| GSE103099 |
Helper-dependent CAV-2 vector-mediated LRRK2G2019S expression in the M. murinus brain induces Parkinson's disease-like histological lesions and motor symptoms |
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