|
| Status |
Public on Nov 08, 2017 |
| Title |
WeightLosers3_T0 |
| Sample type |
RNA |
| |
|
| Source name |
adipose tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Adipose Tissue
gender: Male
time: T0
outcome: WeightLoser
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy Lipid Mini Kit (QIAGEN Nordic, Sollentuna, Sweden), following the manufacturers' instructions. RNA quality was assayed by 2100 Bioanalyzer (Agilent Technologies, Espoo, Finland).
|
| Label |
biotin
|
| Label protocol |
Labelling was done according the the manufacturer's instructions using the GeneChip® 3’ IVT PLUS Protocol. 100ng of total RNAwas reverse transcribed and converted into a double-stranded DNA. In vitro transcription was used to synthesize cRNA and to incorporate biotin-conjugated nucleotides.
|
| |
|
| Hybridization protocol |
A hybridization cocktail is prepared including the labeled, fragmented target and hybridization controls. The target is then hybridized to the GeneChip array in a cartridge format and incubated 16 hours at 45 °C. Immediately following hybridization, the array is washed and stained with streptavidin phycoerythrin conjugate using an automated protocol on the GeneChip® Fluidics Station 450.
|
| Scan protocol |
GeneChip® Scanner
|
| Description |
adipose tissue 16
WeightLoser3 at baseline (T0)
|
| Data processing |
Robust Multi-array Average (RMA) algorithm was used to perform background correction followed by quantile normalization.Brainarray customized Chip Description File version 18.0 (hgu133plus2hsentrezgcdf), was used to map probe sets with corresponding genes.
|
| |
|
| Submission date |
Sep 12, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Miina Ollikainen |
| E-mail(s) |
miina.ollikainen@helsinki.fi
|
| Organization name |
University of Helsinki
|
| Street address |
Tukholmankatu 8
|
| City |
Helsinki |
| ZIP/Postal code |
FI-00014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL19109 |
| Series (2) |
| GSE103766 |
Expression data from adipose tissue of 19 healthy obese individuals at three time-points during one-year weightloss intervention |
| GSE103769 |
One year weight-loss intervention in healthy obese individuals at three time-points |
|
