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Status |
Public on Oct 02, 2008 |
Title |
pUC-18_2 |
Sample type |
RNA |
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Source name |
HEK293 cell line
|
Organism |
Homo sapiens |
Characteristics |
pUC-18 transfected HEK293 cells
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from the cell lines using RNeasy Midi Kit (Quiagen) according to the instructions provided by the manufacturer
|
Label |
DIG-UTP
|
Label protocol |
Digoxigenin-UTP labeled cRNA was generated and amplified from 2 μg of total RNA from each sample using Applied Biosystems NanoAmp RT-IVT Labeling Kit (P/N 4365715) according to the manufacturer’s protocol(P/N 4365710).
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Hybridization protocol |
Array hybridization was performed for 16 hrs at 55 oC. Pre-Hybridization, Hybridization, antibody binding and Chemiluminescence reaction were performed using Applied Biosystems Chemiluminescence Detection Kit (P/N 4342142) following the manufacturer’s protocol (P/N 4339627).
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Scan protocol |
image acquisition and analysis were performed using Applied Biosystems Chemiluminescence Detection Kit (P/N 4342142) and Applied Biosystems 1700 Chemiluminescent Microarray Analyzer (P/N 4338036) following the manufacturer’s protocol (P/N 4339629).
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Description |
pUC-18 transfected HEK293 cells
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Data processing |
Images were auto-gridded and the chemiluminescent signals were quantified, corrected for background, and finally, spot- and spatially-normalized using the Applied Biosystems 1700 Chemiluminescent Microarray Analyzer software v 1.1 (P/N 4336391). Median normalization and Probabilty for a gene to be expressed(A) were calculated with proprietary software.
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Submission date |
Apr 08, 2008 |
Last update date |
Oct 01, 2008 |
Contact name |
Arndt Benecke |
E-mail(s) |
arndt@ihes.fr
|
Phone |
+33160926665
|
Fax |
+33160926609
|
URL |
http://seg.ihes.fr
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Organization name |
Institut des Hautes Etudes Scientifiques & I.R.I.-CNRS
|
Department |
Integrative Biology
|
Lab |
Systems Epigenomics
|
Street address |
35 route des Chartres
|
City |
Bures-sur-Yvette |
ZIP/Postal code |
91440 |
Country |
France |
|
|
Platform ID |
GPL2986 |
Series (1) |
GSE11093 |
EBER2 RNA-induced transcriptome changes identify cellular processes likely targeted during Epstein Barr Virus infection. |
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