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| Status |
Public on Jun 21, 2018 |
| Title |
Resistant strains S6 4 h treatment rep3 |
| Sample type |
SRA |
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| Source name |
Planktonic cultures
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| Organism |
Candida albicans |
| Characteristics |
treatment: 2 µg/ml SM21, 4 h
ploidy: Haploid
genotype: Mutated
phenotype: SM21 resistant
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| Treatment protocol |
No treatment control, 2 µg/ml SM21 (1 h), or 2 µg/ml SM21 (4 h)
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| Growth protocol |
30 °C
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| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNA extraction kit
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
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| Description |
NS64h3
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| Data processing |
Illumina CASAVA v1.8 used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to SC5314 whole genome
FPKM, short for the expected number of Fragments Per Kilobase of transcript sequence per Millions base pairssequenced, is calculated following Trapnell et al., 2010
Genome_build: ASM18296v3
Supplementary_files_format_and_content: .txt file includes FPKM values for each Sample
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| Submission date |
Oct 12, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Thuyen Truong |
| E-mail(s) |
quynhthuyen@gmail.com
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| Organization name |
National University of Singapore
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| Department |
Faculty of Dentistry
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| Street address |
11 Lower Kent Ridge Road
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| City |
Singapore |
| ZIP/Postal code |
119083 |
| Country |
Singapore |
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| Platform ID |
GPL24129 |
| Series (1) |
| GSE104900 |
Transcriptomic analysis of SM21 resistant and susceptible haploid Candida albicans strains |
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| Relations |
| BioSample |
SAMN07779352 |
| SRA |
SRX3276297 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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