|
| Status |
Public on Jan 24, 2018 |
| Title |
H7-Tub-LD1174 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney biopsy from human H7-Tub-LD1174
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Tubulointerstitium from kidney biopsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Rneasy kit (Qiagen) following manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
According to manufacturer's protocols.
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene expression from the tubulointerstitial compartment of human H7-Tub-LD1174
|
| Data processing |
Each batch and platform was processed individually with its corresponding BrainArray CDF and normalized by RMA. After individual batch and platform processing, samples were merged by common EntrezGeneIDs, and finally batch corrected using Combat.
|
| |
|
| Submission date |
Oct 13, 2017 |
| Last update date |
Mar 30, 2022 |
| Contact name |
Sean Eddy |
| E-mail(s) |
seaneddy@umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1150 West Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL22945 |
| Series (1) |
| GSE104954 |
Tubulointerstitial transcriptome from ERCB subjects with chronic kidney disease and living donor biopsies. |
|
