|
| Status |
Public on Dec 04, 2018 |
| Title |
Ime4_dMetll14_rep3 |
| Sample type |
SRA |
| |
|
| Source name |
S2R+ cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
treatment: 10 ug of dsRNA against Ime4 and dMettl14
replicate: 3
passages: 8-10
|
| Treatment protocol |
3 dsRNA treatments (6 days total)
|
| Growth protocol |
S2R+ cells are maintained in Schenider Medium at room temperature
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Cells were pelleted and RNA was isolated with Trizol using standart protocol
Librariesfor sequencing were prepared according to the NEBNext® Ultra™ Directional RNA Library Prep Kit protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
The libraries were sequenced on NextSeq500 with 85 bp paired end
The data was mapped agains Ensembl release 90 using STAR (v.2.5.1b).
Counts per gene were derived using featureCounts (v1.5.1)
Genome_build: BDGP6
Supplementary_files_format_and_content: counts per gene as tab separated file (tsv), read depth normalised bigwig tracks (bw)
|
| |
|
| Submission date |
Oct 23, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jean-Yves Roignant |
| Organization name |
Institute of molecular Biology
|
| Lab |
Roignant
|
| Street address |
Ackermannweg 4
|
| City |
Mainz |
| ZIP/Postal code |
55128 |
| Country |
Germany |
| |
|
| Platform ID |
GPL19132 |
| Series (2) |
| GSE105900 |
RNA-Sequencing of Control KD and KD for Ime4-dMettl14, Fl(2)d, Vir, Nito, CG7358 in S2R+ cells |
| GSE106614 |
Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to other components of the m6A machinery |
|
| Relations |
| BioSample |
SAMN07823323 |
| SRA |
SRX3314152 |
