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Sample GSM2828659 Query DataSets for GSM2828659
Status Public on Dec 04, 2018
Title Vir_rep3
Sample type SRA
 
Source name S2R+ cells
Organism Drosophila melanogaster
Characteristics treatment: 10 ug of dsRNA against Vir
replicate: 3
passages: 8-10
Treatment protocol 3 dsRNA treatments (6 days total)
Growth protocol S2R+ cells are maintained in Schenider Medium at room temperature
Extracted molecule polyA RNA
Extraction protocol Cells were pelleted and RNA was isolated with Trizol using standart protocol
Librariesfor sequencing were prepared according to the NEBNext® Ultra™ Directional RNA Library Prep Kit protocol.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing The libraries were sequenced on NextSeq500 with 85 bp paired end
The data was mapped agains Ensembl release 90 using STAR (v.2.5.1b).
Counts per gene were derived using featureCounts (v1.5.1)
Genome_build: BDGP6
Supplementary_files_format_and_content: counts per gene as tab separated file (tsv), read depth normalised bigwig tracks (bw)
 
Submission date Oct 23, 2017
Last update date May 15, 2019
Contact name Jean-Yves Roignant
Organization name Institute of molecular Biology
Lab Roignant
Street address Ackermannweg 4
City Mainz
ZIP/Postal code 55128
Country Germany
 
Platform ID GPL19132
Series (2)
GSE105900 RNA-Sequencing of Control KD and KD for Ime4-dMettl14, Fl(2)d, Vir, Nito, CG7358 in S2R+ cells
GSE106614 Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to other components of the m6A machinery
Relations
BioSample SAMN07823317
SRA SRX3314158

Supplementary file Size Download File type/resource
GSM2828659_imb_roignant_2017_20_12_Vir_rep3_S12.readcounts.tsv.gz 71.9 Kb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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