|
Status |
Public on Sep 04, 2018 |
Title |
BH150464-2_JS70020 3-Ca |
Sample type |
RNA |
|
|
Source name |
BH150464-2
|
Organism |
Homo sapiens |
Characteristics |
disease: squamous cell carcinoma (SCC) tissue: tumor tissue type: fresh tissue
|
Treatment protocol |
Two types of samples from 3 pairs of tumor/adjacent normal tissue paraffin specimens and 1 pair of tumor/adjacent normal fresh tissue samples.
|
Growth protocol |
Tissues from HIV associated lung cancer
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
Ca-tumor-fresh
|
Data processing |
The data were nomanized by RMA with Parteck Genomic Suit 7.1
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|
|
Submission date |
Nov 15, 2017 |
Last update date |
Sep 05, 2018 |
Contact name |
WeiPing Chen |
E-mail(s) |
weipingChen@niddk.nih.gov
|
Phone |
301-496-0175
|
Organization name |
NIDDK/NIH
|
Department |
GCL
|
Lab |
Genomics Core Lab
|
Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE106937 |
Molecular Changes of Lung Malignancy in HIV Infection |
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