|
| Status |
Public on May 10, 2008 |
| Title |
Differentiated basal Biological Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Differentiated basal Cells
|
| Organism |
Homo sapiens |
| Characteristics |
Human reduction mammoplasty sample, 26 year old patient
|
| Treatment protocol |
Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
|
| Growth protocol |
Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
|
| Label |
biotin
|
| Label protocol |
50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
|
| |
|
| Hybridization protocol |
cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
|
| Scan protocol |
GeneChip® Scanner 3000 7G, Affymetrix
|
| Description |
Gene expression data from differentiated basal subpopulation of mammary epithelial cells
|
| Data processing |
The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
|
| |
|
| Submission date |
May 09, 2008 |
| Last update date |
May 09, 2008 |
| Contact name |
BCGSC BC Cancer Agency |
| E-mail(s) |
support@mouseatlas.org
|
| Organization name |
Canada's Michael Smith Genome Sciences Centre
|
| Department |
Gene Expression
|
| Lab |
Marco Marra
|
| Street address |
675 West 10th Avenue
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1352 |
| Series (2) |
| GSE11394 |
Transcript Analysis of Human Mammary Cell Subsets by Chip Technology |
| GSE11395 |
Transcript Analysis of Human Mammary Cell Subsets |
|
