|
| Status |
Public on Dec 01, 2008 |
| Title |
Saccharomyces cerevisiae; alpha-factor arrested |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from alpha-factor arrested cells
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain info: MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 bar1Δ::KanMX
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using hot acidic phenol. 50 ug of total RNA was treated with Turbo Dnase (Ambion), and checked for integrity using a Bioanalyzer 2100 (Agilent).
|
| Label |
biotin
|
| Label protocol |
According to Affymetrix protocol included with Yeast 2.0 arrays.
|
| |
|
| Hybridization protocol |
According to Affymetrix protocol included with Yeast 2.0 arrays.
|
| Scan protocol |
According to Affymetrix protocol included with Yeast 2.0 arrays.
|
| Description |
Total RNA extracted from alpha-factor arrested cells.
|
| Data processing |
Raw data was normalized using the affy package from Bioconductor.
|
| |
|
| Submission date |
May 11, 2008 |
| Last update date |
Jun 21, 2008 |
| Contact name |
Jay R. Hesselberth |
| E-mail(s) |
jay.hesselberth@cuanschutz.edu
|
| Organization name |
University of Colorado School of Medicine
|
| Department |
Biochemistry and Molecular Gentetics
|
| Lab |
Jay Hesselberth
|
| Street address |
12801 E 17TH AVE
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE11412 |
Alpha-factor treatment of Saccharomyces cerevisiae |
|
