|
| Status |
Public on Dec 13, 2019 |
| Title |
SubcutaneousAdiposeTissueFamily22Twin2 |
| Sample type |
RNA |
| |
|
| Source name |
Subcutaneous Adipose Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
individual: Family22Twin2
gender: female
age: 36
bmi category: lean
tissue: SAT
|
| Treatment protocol |
Total RNA extract treated with DNase I (Qiagen)
|
| Growth protocol |
SAT biopsies via a surgical technique under local anaesthesia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Lipid Tissue Mini Kit (Qiagen) to extract SAT RNA
|
| Label |
biotin
|
| Label protocol |
biotin
|
| |
|
| Hybridization protocol |
hybridized 250ng to Affymetrix U133 Plus 2.0 microarray
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner
|
| Data processing |
GCRMA method used to normalize data
|
| |
|
| Submission date |
Dec 15, 2017 |
| Last update date |
Dec 13, 2019 |
| Contact name |
Maheswary Muniandy |
| E-mail(s) |
maheswary.muniandy@helsinki.fi
|
| Organization name |
University Helsinki
|
| Street address |
Tukholmankatu 8
|
| City |
Helsinki |
| ZIP/Postal code |
00270 |
| Country |
Finland |
| |
|
| Platform ID |
GPL19109 |
| Series (1) |
| GSE92405 |
Gene expression profile of subcutaneous adipose tissue in BMI-discordant monozygotic twin pairs in obesity |
|
