|
| Status |
Public on Jun 21, 2018 |
| Title |
28-2: MT1 |
| Sample type |
SRA |
| |
|
| Source name |
otic tissue
|
| Organism |
Sus scrofa |
| Characteristics |
tissue: inner ear
developmental stage: 28-day old embryo
genotype: SOX10 p.R109W mutation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Otic vesicles were obtained from both wild-type and mutant embryos at E28,total RNA was extracted using Trizol reagent(Invitrogen,CA, USA)following the manufacturer's protocol.
RNA libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
mapping fastq to sus_scrofa ensemble release 85 with STAR
caculate read counts via htseq
Genome_build: sus_scrofa ensemble release 85
Supplementary_files_format_and_content: raw counts from htseq2
|
| |
|
| Submission date |
Feb 04, 2018 |
| Last update date |
Jun 21, 2018 |
| Contact name |
hao qingqing |
| E-mail(s) |
gwent001@163.com
|
| Organization name |
Chinese PLA Medical School, China
|
| Street address |
Chinese PLA Medical School, Beijng
|
| City |
beijing |
| ZIP/Postal code |
100853 |
| Country |
China |
| |
|
| Platform ID |
GPL24486 |
| Series (1) |
| GSE110103 |
RNA expression data of inner ear tissues in SOX10 p.R109W mutation pigs with inner ear malformation and wild-type pigs |
|
| Relations |
| BioSample |
SAMN08462924 |
| SRA |
SRX3645827 |
