|
| Status |
Public on Dec 22, 2008 |
| Title |
human endometrium time 0 human array replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
human endometrium time 0 human array replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
time 0 control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and Qiagen RNeasy purification were performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on GeneChipHuman U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip 2500 Scanner (Affymetrix).
|
| Description |
human on human control
|
| Data processing |
MAS5.0 was used for initial data extraction, raw data normalised to the internal spikes using R. Human and mouse orthologues and homologues were identified using Netaffix and only those transcripts used for further analysis.
|
| |
|
| Submission date |
Jun 12, 2008 |
| Last update date |
Mar 16, 2009 |
| Contact name |
D. Stephen Charnock-Jones |
| E-mail(s) |
dscj1@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Obstetrics and Gynaecology
|
| Street address |
The Rosie Hospital
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0SW |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL8300 |
| Series (2) |
| GSE11768 |
Nude mouse model of endometriosis |
| GSE11769 |
Analysis of ectopic human endometrium and peritoneal tissues in nude mice |
|
