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Sample GSM299781 Query DataSets for GSM299781
Status Public on Jul 23, 2008
Title Primary aortic endothelial cells, transgenic_PPARG_V290M_1
Sample type RNA
 
Source name Primary aortic endothelial cells, transgenic_PPARG_V290M
Organism Mus musculus
Characteristics Mouse: strain C57
Treatment protocol No treatment
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol.
Description Gene expression data from primary aortic endothelial cells.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 24, 2008
Last update date Aug 28, 2018
Contact name Henry Keen
E-mail(s) henry-keen@uiowa.edu
Phone 319-335-8926
Organization name University of Iowa
Department Pharmacology
Lab Dr. Curt D. Sigmund's Laboratory
Street address 2-370 BSB
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Platform ID GPL1261
Series (1)
GSE11870 Gene expression changes in primary aortic endothelial cells during expression of dominant negative PPAR gamma (V290M).
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 3550.8 P
AFFX-BioB-M_at 5923.4 P
AFFX-BioB-3_at 4807.7 P
AFFX-BioC-5_at 10267.3 P
AFFX-BioC-3_at 7927.6 P
AFFX-BioDn-5_at 18126.1 P
AFFX-BioDn-3_at 23448.1 P
AFFX-CreX-5_at 48504.7 P
AFFX-CreX-3_at 46106.9 P
AFFX-DapX-5_at 22.5 A
AFFX-DapX-M_at 6.2 A
AFFX-DapX-3_at 29.6 A
AFFX-LysX-5_at 17.7 A
AFFX-LysX-M_at 44.5 A
AFFX-LysX-3_at 8.1 A
AFFX-PheX-5_at 2.9 A
AFFX-PheX-M_at 21.7 A
AFFX-PheX-3_at 14.5 A
AFFX-ThrX-5_at 6.4 A
AFFX-ThrX-M_at 5.8 A

Total number of rows: 45101

Table truncated, full table size 850 Kbytes.




Supplementary file Size Download File type/resource
GSM299781.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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