|
| Status |
Public on Aug 22, 2008 |
| Title |
Growing cells, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Growing activated hepatic stellate cells
|
| Organism |
Homo sapiens |
| Characteristics |
fibrogenic cells of the liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAeasy Qiagen kit, according manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip scanner 3000.
|
| Description |
gene expression data from growing cells
|
| Data processing |
data was analysed with ArrayAssist (Invitrogen), normalization was performed using GC-RMA algorithm
|
| |
|
| Submission date |
Jul 01, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Valery Krizhanovsky |
| E-mail(s) |
krizh@cshl.edu
|
| Phone |
516-367-8408
|
| Organization name |
Cold Spring harbor Laboratory
|
| Lab |
Lowe lab
|
| Street address |
1 Bungtown Rd
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE11954 |
Expression analysis of growing and senescent activated hepatic stellate cells |
|
| Relations |
| Reanalyzed by |
GSE119087 |
