|
| Status |
Public on Nov 21, 2008 |
| Title |
B. thetaiotaomicron responses to purified host glycans and components: core 1 disaccharide 1 |
| Sample type |
RNA |
| |
|
| Source name |
bacterial
|
| Organism |
Bacteroides thetaiotaomicron |
| Characteristics |
strain VPI-5482
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy mini column and Ambion DNAfree DNAseI digestion
|
| Label |
biotin
|
| Label protocol |
Enzo Biochemicals Bioarray terminal labeling kit
|
| |
|
| Hybridization protocol |
Affymetrix
|
| Scan protocol |
Affymetrix
|
| Description |
B. thetaiotaomicron grown in minimal medium plus 0.5% core 1 disaccharide (galactose-beta-1,3-N-acteylgalactosamine), 5ml culture in an anaerobic tube. Replicate 1 of 2.
|
| Data processing |
Data values for B. thetaiotamicron genes normalized to 500 in Affymetrix MAS5 software (excluded other species that were on chip using electronic mask)
|
| |
|
| Submission date |
Jul 01, 2008 |
| Last update date |
Nov 21, 2008 |
| Contact name |
Eric Charles Martens |
| E-mail(s) |
emartens@umich.edu
|
| Phone |
734-647-5800
|
| Organization name |
University of Michigan Medical School
|
| Department |
Dept. of Microbiology and Immunology
|
| Street address |
1150 West Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL7006 |
| Series (2) |
| GSE11962 |
Growth of B. thetaiotaomicron on purified host mucosal glycans and glycan fragments |
| GSE11980 |
Glycan effect on Saccharolytic Human Distal Gut Symbiont, B. thetaiotaomicron |
|
