|
Status |
Public on Nov 21, 2008 |
Title |
B. thetaiotaomicron responses to purified host glycans and components: core 1 disaccharide 2 |
Sample type |
RNA |
|
|
Source name |
bacterial
|
Organism |
Bacteroides thetaiotaomicron |
Characteristics |
strain VPI-5482
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNAeasy mini column and Ambion DNAfree DNAseI digestion
|
Label |
biotin
|
Label protocol |
Enzo Biochemicals Bioarray terminal labeling kit
|
|
|
Hybridization protocol |
Affymetrix
|
Scan protocol |
Affymetrix
|
Description |
B. thetaiotaomicron grown in minimal medium plus 0.5% core 1 disaccharide (galactose-beta-1,3-N-acteylgalactosamine), 5ml culture in an anaerobic tube. Replicate 2 of 2.
|
Data processing |
Data values for B. thetaiotamicron genes normalized to 500 in Affymetrix MAS5 software (excluded other species that were on chip using electronic mask)
|
|
|
Submission date |
Jul 01, 2008 |
Last update date |
Nov 21, 2008 |
Contact name |
Eric Charles Martens |
E-mail(s) |
emartens@umich.edu
|
Phone |
734-647-5800
|
Organization name |
University of Michigan Medical School
|
Department |
Dept. of Microbiology and Immunology
|
Street address |
1150 West Medical Center Drive
|
City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL7006 |
Series (2) |
GSE11962 |
Growth of B. thetaiotaomicron on purified host mucosal glycans and glycan fragments |
GSE11980 |
Glycan effect on Saccharolytic Human Distal Gut Symbiont, B. thetaiotaomicron |
|