|
| Status |
Public on Dec 19, 2008 |
| Title |
SG-107A_phenotype of EPC colony assay |
| Sample type |
RNA |
| |
|
| Source name |
cultured EPC, baseline
|
| Organism |
Homo sapiens |
| Characteristics |
baseline
|
| Treatment protocol |
cells were harvested after 5 days.
|
| Growth protocol |
Blood was collected into CPT tubes with Ficoll and sodium heparin for isolation of mononuclear cells, washed twice in PBS with 5% fetal bovine serum and re-suspended in media for endothelial progenitor-colony-forming assay.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cultured EPC with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
|
| Label |
biotin
|
| Label protocol |
Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
|
| Scan protocol |
Affymetrix GeneChip scanner
|
| Description |
EPC sampled from blood and cultured
|
| Data processing |
Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation
|
| |
|
| Submission date |
Jul 17, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Richard Cannon |
| E-mail(s) |
cannonr@nih.gov
|
| Phone |
301-496-9895
|
| Organization name |
NIH
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-1454 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE12155 |
Characterization of a Colony Assay Used to Investigate Endothelial Progenitor Cells |
|
| Relations |
| Reanalyzed by |
GSE119087 |
