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Sample GSM30663 Query DataSets for GSM30663
Status Public on Jul 15, 2005
Title Comparison wt vs aft1 experiment 1
Sample type RNA
 
Channel 1
Source name CM3260 (wt) minus iron
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name Y18 (aft1) minus iron
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Strains were grown exponentially in Bio101#4027-122, and total RNA was extracted using the hot phenol method. Yeast Genefilters Microarrays were used to hybridize the resulting RNA according to the manufacturer's instructions. Five micrograms of total RNA were reverse-transcribed, and hybridizations were done in duplicate using two separate GeneFilters. The membranes were exposed to a phosphor imaging screen for 3 days. Images were acquired by using a Storm 860 (Molecular Dynamics), and analyzed by using the PATHWAYS 3 (Research Genetics) software.
Keywords = iron metabolism
Keywords = transcription factor
 
Submission date Sep 15, 2004
Last update date May 28, 2005
Contact name Pierre-Louis Blaiseau
E-mail(s) blaiseau@ijm.jussieu.fr
Phone (33) 1 44 27 4741
Organization name Institute Jacques Monod
Department
Lab Protein Engineering and Metabolic Control
Street address 2 Place Jussieu
City Paris
ZIP/Postal code F-75251
Country France
 
Platform ID GPL205
Series (1)
GSE1763 Transcriptional effect of aft1 and aft2 mutation in iron depleted conditions

Data table header descriptions
ID_REF
VALUE log ratio (log2 of prevalue)
PREVALUE CH1/CH2
NORMCH1 Normalized signal 1
NORMCH2 Normalized signal 2

Data table
ID_REF VALUE PREVALUE NORMCH1 NORMCH2
1 -0.37 0.77 153.13 198.28
2 0.41 1.33 262.9 198.34
3 0.35 1.27 1287.43 1010.14
4 -0.96 0.51 51.3 99.89
5 -1.41 0.38 1468.99 3916.99
6 0.53 1.44 612.07 423.69
7 -0.35 0.79 162.26 206.47
8 -0.38 0.77 134.09 174.59
9 -0.25 0.84 80 95.35
10 0.08 1.06 192.31 181.53
11 -0.91 0.53 420.69 792.59
12 -0.84 0.56 67.95 121.26
13 -0.6 0.66 277.86 421.5
14 0.2 1.15 135.7 118.39
15 -0.75 0.59 463.9 781.5
16 -0.19 0.88 210.47 240.31
17 0.22 1.16 97 83.36
18 0.14 1.1 292.52 265.98
19 -0.21 0.86 424.53 492.21
20 -0.3 0.81 90.51 111.23

Total number of rows: 6084

Table truncated, full table size 168 Kbytes.




Supplementary data files not provided
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