|
| Status |
Public on Mar 04, 2019 |
| Title |
cry_INPUT |
| Sample type |
SRA |
| |
|
| Source name |
cell
|
| Organism |
Schizosaccharomyces cryophilus |
| Characteristics |
tissue: whole cells
strain: wild-type
chip antibody: INPUT
|
| Growth protocol |
cell were gwon in rich medium (YES).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were prepared using standard bead beating procedure, sonicated to achieve fragmements of 200-400bp and clarified by centrifugation at 17000g.
Libraries were prepared using standard Illumina pipeline.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
cry_INPUT
|
| Data processing |
ChIP-seq reads were aligned to reference genome using Bowtie2
Genome_build: Pombe: ASM294v2.20
Genome_build: S. japonicus: GCA_000149845
Genome_build: S. croyphylus: assembly genome
Genome_build: S. octosporus: assembly genome
Supplementary_files_format_and_content: bigwig files were generated deeptools normalized by RPKM
|
| |
|
| Submission date |
Mar 28, 2018 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Robin Allshire |
| E-mail(s) |
Robin.Allshire@ed.ac.uk
|
| Organization name |
The University of Edinburgh
|
| Lab |
Allshire Lab
|
| Street address |
Max Born Cresent
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH9 3BF |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL24777 |
| Series (1) |
| GSE112454 |
Fission yeasts CENPA and H3K9me Chip-seq |
|
| Relations |
| BioSample |
SAMN08811359 |
| SRA |
SRX3858790 |
