|
Status |
Public on Mar 04, 2019 |
Title |
pombe_h90-1_H3K9_PE |
Sample type |
SRA |
|
|
Source name |
cell
|
Organism |
Schizosaccharomyces pombe |
Characteristics |
tissue: whole cells strain: pombe_h90 chip antibody: H3K9
|
Growth protocol |
cell were gwon in rich medium (YES).
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Lysates were prepared using standard bead beating procedure, sonicated to achieve fragmements of 200-400bp and clarified by centrifugation at 17000g. Libraries were prepared using standard Illumina pipeline.
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
pombe_h90-1_H3K9_PE
|
Data processing |
ChIP-seq reads were aligned to reference genome using Bowtie2 Genome_build: Pombe: ASM294v2.20 Genome_build: S. japonicus: GCA_000149845 Genome_build: S. croyphylus: assembly genome Genome_build: S. octosporus: assembly genome Supplementary_files_format_and_content: bigwig files were generated deeptools normalized by RPKM
|
|
|
Submission date |
Mar 28, 2018 |
Last update date |
Mar 04, 2019 |
Contact name |
Robin Allshire |
E-mail(s) |
Robin.Allshire@ed.ac.uk
|
Organization name |
The University of Edinburgh
|
Lab |
Allshire Lab
|
Street address |
Max Born Cresent
|
City |
Edinburgh |
State/province |
Midlothian |
ZIP/Postal code |
EH9 3BF |
Country |
United Kingdom |
|
|
Platform ID |
GPL17225 |
Series (1) |
GSE112454 |
Fission yeasts CENPA and H3K9me Chip-seq |
|
Relations |
BioSample |
SAMN08811464 |
SRA |
SRX3858812 |