|
| Status |
Public on Dec 30, 2011 |
| Title |
2MLN_dnTbRII |
| Sample type |
RNA |
| |
|
| Source name |
Xenografted 2MLN-dnTbRII tumors.
|
| Organism |
Homo sapiens |
| Characteristics |
total rna: extracted from xenografted 2MLN-dnTbRII tumors.
|
| Treatment protocol |
2MLN-dnTbRII cells were injected subcutaneously at the left flank of male Balb/c nude mice of 4-5 weeks.
|
| Growth protocol |
Tumors were allowed to grow for one week.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tumor tissue was digested with 1 mg/ml collagenase (Worthington, NJ, USA) and 0,25% Trypsin-EDTA (Invitrogen). The resulting single cell suspension was subjected to MACS cell separation using microbeads conjugated to CD326 antibody (Miltenyi Biotec, CA, USA) allowing the selection of CD326 expressing human cancer cells from CD326 negative mouse stromal cells. Total RNA from the isolated cancer cells were purified using the RNeasy Mini Kit (QIAGEN).
|
| Label |
Biotin-labelled.
|
| Label protocol |
According to Affymetrix's instructions.
|
| |
|
| Hybridization protocol |
According to Affymetrix's instructions.
|
| Scan protocol |
According to Affymetrix's instructions.
|
| Description |
Gene expression analysis of TGF-beta signaling-disrupting gastric cancer cell line 2MLN.
|
| Data processing |
MAS5.0
|
| |
|
| Submission date |
Aug 05, 2008 |
| Last update date |
Dec 30, 2011 |
| Contact name |
Mitsunobu R Kano |
| E-mail(s) |
mikano-tky@umin.net
|
| Phone |
+81-3-5841-3356
|
| Fax |
+81-3-5841-3354
|
| Organization name |
The University of Tokyo
|
| Department |
Graduate School of Medicine
|
| Lab |
Department of Molecular Pathology
|
| Street address |
Hongo 7-3-1
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE12336 |
Gene expression analysis of TGF-beta signaling-disrupting gastric cancer cell line 2MLN |
|
