|
| Status |
Public on Oct 15, 2018 |
| Title |
WRKY19 OE Sultana_4 |
| Sample type |
RNA |
| |
|
| Source name |
WRKY19 overexpressing plantlet of Vitis vinifera cv. Sultana_line 4
|
| Organism |
Vitis vinifera |
| Characteristics |
cultivar: Sultana
genotype/variation: WRKY19 overexpressing
tissue: young leaves
|
| Treatment protocol |
For transient transformation of Vitis vinifera cv. Sultana, the 35S:VvWRKY19 construct was transferred to Agrobacterium tumefaciens strain C58C1 by electroporation. As control, Agrobacterium was also transformed with an empty GoldenBraid 2.0 vector (pDGBΩ1). Seven in vitro six-week-old plants of grapevine cv. Sultana were immersed in each bacterial suspension and vacuum infiltrated (2 X 2 min at 90kPa).
|
| Growth protocol |
For the transient overexpression of VvWRKY19 in grapevine, plantlets of Vitis vinifera cv. Sultana were in vitro micropropagated and cultivated in growth chamber at 25°C with a 16-h photoperiod.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from ~100 mg of young and well expanded ground leaves using SpectrumTM Plant Total RNA kit (Sigma-Aldrich) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
For microarray analysis on Sultana leaves transiently overexpressing VvWRKY19, the cDNA synthesis and labeling reactions have been conducted according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).
|
| |
|
| Hybridization protocol |
For microarray analysis on Sultana leaves transiently overexpressing VvWRKY19, the hybridization and washing reactions have been conducted according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).
|
| Scan protocol |
Scanning and Feature Extraction were performed by using an Agilent Scanner following the settings and parameters indicated in the instruction manual.
|
| Data processing |
A data-matrix was prepared selecting from each single sub-array outcome file the gProcessedSignalvalues, which are the raw fluorescence intensities of each probe.
The data were normalized on the 75th percentile and a correlation analysis was then performed to assess the consistency of the biological quadruplicates.
|
| |
|
| Submission date |
Apr 16, 2018 |
| Last update date |
Oct 15, 2018 |
| Contact name |
Silvia Dal Santo |
| E-mail(s) |
silvia.dalsanto@univr.it
|
| Organization name |
University of Verona
|
| Department |
Department of Biotechnology
|
| Street address |
Strada Le Grazie, 15
|
| City |
Verona |
| ZIP/Postal code |
37134 |
| Country |
Italy |
| |
|
| Platform ID |
GPL22427 |
| Series (2) |
| GSE113223 |
Timing and order of the molecular events marking the onset of berry ripening in grapevine [WRKY19 OE] |
| GSE113225 |
Timing and order of the molecular events marking the onset of berry ripening in grapevine |
|
