Muscle biopsies were obtained from the lateral portion of the vastus lateralis muscle, 10-20 cm above the knee. The biopsies were obtained using a Bergström biopsy needle after local anaesthesia in the ICU patients and just after sedation in the control patients.
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from muscle samples using TRIzol (Invitrogen, Stockholm, Sweden) and quantified using a Nano-drop spectrophotometer.
Label
biotin
Label protocol
Total RNA was labelled, converted to cRNA and fragmented as recommended by Affymetrix.
Hybridization protocol
As Affymetrix
Scan protocol
As Affymetrix
Description
none
Data processing
Data presented in the paper was quality controlled using the Microarray Suite software (MAS 5.0). All array data were normalized through implementations of the MAS5 algorithm, to a global scaling intensity of 100. Probesets declared absent by MAS5 across all chips were removed from further analysis.
