|
| Status |
Public on Oct 23, 2008 |
| Title |
ALL_ resistant_11+IR |
| Sample type |
RNA |
| |
|
| Source name |
ALL, resistant, irradiated
|
| Organism |
Homo sapiens |
| Characteristics |
B-precursor ALL tumour
resistant to IR-induced DNA damage
|
| Treatment protocol |
Cells were irradiated and maintained in culture for 8 hours, or maintained for 8 h without irradiation.
|
| Growth protocol |
Mononuclear cells were isolated from bone marrow aspirate using Ficoll-Paque (Amersham Biosciences, Piscataway, NJ), and cells were cultured in RPMI 1640. Only samples with more than 90% of blast cells were used for analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard Affymetrix protocol
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
122_8h
Aliquots of 2 x 106 cells per time point were irradiated with 5 Gy IR (cobalt Co60)
|
| Data processing |
Data for all arrays was normalised in the GeneSpring GX 7.3.1 analysis package to the 50th percentile of total expression value data for each individual chip, and to the median for each gene across arrays (default parameters).
|
| |
|
| Submission date |
Oct 20, 2008 |
| Last update date |
Oct 23, 2008 |
| Contact name |
Carmel M McConville |
| E-mail(s) |
c.mcconville@bham.ac.uk
|
| Organization name |
University of Birmingham
|
| Department |
School of Cancer Sciences
|
| Street address |
Vincent Drive
|
| City |
Birmingham |
| State/province |
UK |
| ZIP/Postal code |
B15 2TT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE13280 |
Gene expression analysis of paediatric acute lymphoblastic leukaemia |
|
