|
| Status |
Public on Oct 30, 2018 |
| Title |
AFB168 |
| Sample type |
genomic |
| |
|
| Source name |
CD14+ Monocytes, resting cells
|
| Organism |
Homo sapiens |
| Characteristics |
age: 40.76
gender: Male
batch: 1
ethnicity: African descent
|
| Treatment protocol |
Primary monocytes were positively selected with magnetic CD14 microbeads from Miltenyi Biotec, according to the manufacturer's instructions
|
| Growth protocol |
Monocyte DNA samples were obtained from PBMCs frozen in 90% fetal calf serum (FCS) and 10% dimethyl sulfoxide dimethyl sulfoxid
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Each DNA sample was extracted from 2x106 primary monocytes by a standard phenol/chloroform protocol followed by ethanol precipitation. Prior to DNA methylation profiling, DNAs were then bisulfite converted and processed using the Illumina Infinium MethylationEPIC BeadChip Kit, according to the manufacturer's instructions followed by ethanol precipitation
|
| Label |
cy3,cy5
|
| Label protocol |
Sample barcoding and staining were carried out by following the instructions of Illumina provided in the Infinium MethylationEPIC BeadChip Kit
|
| |
|
| Hybridization protocol |
Bisulfite converted DNAs were hybridized to beadchips provided the Illumina Infinium MethylationEPIC BeadChip Kit
|
| Scan protocol |
Signal intensity is measured with the Illumina HiScan system to generate beta values, a measure of the degree of methylation at a locus
|
| Description |
SAMPLE 64
|
| Data processing |
Signal intensity is measured with the Illumina HiScan system to generate beta values, a measure of the degree of methylation at a locus
|
| |
|
| Submission date |
Sep 28, 2018 |
| Last update date |
Oct 30, 2018 |
| Contact name |
Lucas Theo Husquin |
| E-mail(s) |
lucas.husquin@pasteur.fr
|
| Organization name |
Institut Pasteur
|
| Department |
Genetics and Genomics
|
| Lab |
Human Evolutionary Genetics
|
| Street address |
28 rue du docteur Roux
|
| City |
Paris |
| State/province |
--- Select a state --- |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
|
| Platform ID |
GPL23976 |
| Series (1) |
| GSE120610 |
Exploring the Genetic Basis of Human Population Differences in DNA Methylation and their Causal Impact on Immune Gene Regulation |
|
